Objective: To develop a reliable method for the determination of tetrahydrobiopterin and total biopterin in plasma. Methods: Modifications include blood collection and handling procedures. Results: Collection of blood in the presence of 0.1% (w/v) dithiothreitol and waiting for 2-3 h before centrifugation are optimal for adequate quantitation of both pterins. Conclusions: For a reliable determination of tetrahydrobiopterin and total biopterin in plasma, blood tubes must contain a dimercapto-antioxidant and time between blood collection and centrifugation is critical.

Biopterin, HPLC, Humans, Plasma, Tetrahydrobiopterin
dx.doi.org/10.1016/j.clinbiochem.2006.12.001, hdl.handle.net/1765/35551
Clinical Biochemistry
Erasmus MC: University Medical Center Rotterdam

Fekkes, D, & Voskuilen-Kooijman, A. (2007). Quantitation of total biopterin and tetrahydrobiopterin in plasma. Clinical Biochemistry, 40(5-6), 411–413. doi:10.1016/j.clinbiochem.2006.12.001