2007-03-01
Quantitation of total biopterin and tetrahydrobiopterin in plasma
Publication
Publication
Clinical Biochemistry , Volume 40 - Issue 5-6 p. 411- 413
Objective: To develop a reliable method for the determination of tetrahydrobiopterin and total biopterin in plasma. Methods: Modifications include blood collection and handling procedures. Results: Collection of blood in the presence of 0.1% (w/v) dithiothreitol and waiting for 2-3 h before centrifugation are optimal for adequate quantitation of both pterins. Conclusions: For a reliable determination of tetrahydrobiopterin and total biopterin in plasma, blood tubes must contain a dimercapto-antioxidant and time between blood collection and centrifugation is critical.
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| doi.org/10.1016/j.clinbiochem.2006.12.001, hdl.handle.net/1765/35551 | |
| Clinical Biochemistry | |
| Organisation | Erasmus MC: University Medical Center Rotterdam |
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Fekkes, D., & Voskuilen-Kooijman, A. (2007). Quantitation of total biopterin and tetrahydrobiopterin in plasma. Clinical Biochemistry, 40(5-6), 411–413. doi:10.1016/j.clinbiochem.2006.12.001 |
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