Surgical handling of the peritoneum causes an inflammatory reaction, during which a potentially lethal cocktail of active mediators is produced, including cytokines and growth factors. The aim of this study was to investigate the effects of inflammatory cytokines on the interaction between tumor and mesothelial cells. Tumor cell adhesion to a mesothelial monolayer was assessed after preincubation of the mesothelium with interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Preincubation of the mesothelial monolayer with IL-1β or TNF-α resulted in enhanced tumor cell adhesion of Caco2 and HT29 colon carcinoma cells. The amount of stimulation for the Caco2 cells was between 20% and 40% and for HT29 cells between 30% and 70%. Blocking experiments with anti-IL-1β and anti-TNF-α resulted in significant inhibition of the cytokine-stimulated tumor cell adhesion. The presented results prove that IL-1β and TNF-α are significant stimulating factors in tumor cell adhesion in vitro and may therefore account for tumor recurrence to the peritoneum in vivo.

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Digestive Diseases and Sciences
Erasmus MC: University Medical Center Rotterdam

van Grevenstein, H., Hofland, L., van Rossen, M. E., van Koetsveld, P., Jeekel, H., & van Eijck, C. (2007). Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Digestive Diseases and Sciences, 52(10), 2775–2783. doi:10.1007/s10620-007-9778-4