We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used.

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doi.org/10.1016/j.mimet.2007.01.013, hdl.handle.net/1765/35802
Journal of Microbiological Methods
Erasmus MC: University Medical Center Rotterdam

Melles, D.C, van Leeuwen, W.B, Snijders, S.V, Horst-Kreft, D, Peeters, J.K, Verbrugh, H.A, & van Belkum, A.F. (2007). Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus. Journal of Microbiological Methods, 69(2), 371–375. doi:10.1016/j.mimet.2007.01.013