Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

Melles, Damian; van Leeuwen, Willem; Snijders, Susan; Horst-Kreft, Deborah; Peeters, Justine; Verbrugh, Henri; van Belkum, Alex

doi:10.1016/j.mimet.2007.01.013

D.C. Melles (Damian), W.B. van Leeuwen (Willem), S.V. Snijders (Susan), D. Horst-Kreft (Deborah), J.K. Peeters (Justine), H.A. Verbrugh (Henri) and A.F. van Belkum (Alex)

2007-05-01

Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

Journal of Microbiological Methods , Volume 69 - Issue 2 p. 371- 375

We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used.

Additional Metadata
Keywords	AFLP, MLST, Molecular typing, PFGE, Staphylococcus aureus
Persistent URL	doi.org/10.1016/j.mimet.2007.01.013, hdl.handle.net/1765/35802
Journal	Journal of Microbiological Methods
Organisation	Erasmus MC: University Medical Center Rotterdam

Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

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Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

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