TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients. The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.

Cancer cell lines, Colon cancer, Splice variants, TIMP-1
dx.doi.org/10.1016/j.molonc.2007.05.002, hdl.handle.net/1765/36976
Molecular Oncology
Erasmus MC: University Medical Center Rotterdam

Usher, P.A, Sieuwerts, A.M, Bartels, A, Lademann, U, Nielsen, H.J, Holten-Andersen, L, … Offenberg, H. (2007). Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue. Molecular Oncology, 1(2), 205–215. doi:10.1016/j.molonc.2007.05.002