Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (-0.5 mg dry weight) of human and pig cardiac tissue.

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Proteomics - Clinical Applications
Erasmus MC: University Medical Center Rotterdam

Zaremba, R., Merkus, D., Hamdani, N., Lamers, J., Paulus, W., dos Remedios, C., … van der Velden, J. (2007). Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies. Proteomics - Clinical Applications, 1(10), 1285–1290. doi:10.1002/prca.200600891