A human monoclonal antibody type IgG4, designated 1Ff4, was obtained by Epstein Barr virus transformation of peripheral blood lymphocytes from a hepatitis B vaccinee (HB-VAX: plasma-derived vaccine) after one boost of yeast recombinant DNA derived vaccine (Engerix-B). 1Ff4 binds preferentially to HBsAg/adw(2) and HBsAg/ayw(1). In binding experiments, it competes with antibodies induced by vaccination with HB-VAX-DNA (yeast recombinant) and HB-VAX (plasma-derived vaccine). 1Ff4 competes in part with a monoclonal antibody for the w/r region. Partial inhibition of binding of HBsAg/adw(2) to solid phase anti-HBs was detected, resembling inhibition obtained using other human monoclonal specific for the "a"-loop. 1Ff4 does not bind to linear peptides covering the two "a"-loops or to an adw(2)/G145R mutant, its binding to wild type HBsAg strongly depends on the presence of disulphide bonds. In a large series of HBsAg-positive samples from an endemic area, 1Ff4 antibodies were successfully used to discriminate between an adw(2) and an adrq+ strain. The characterisation of 1Ff4 and other human monoclonal anti-HBs antibodies may help to understand the fine specificity of protective antibodies elicited by immunization.

Antibodies, Monoclonal/*immunology, Binding, Competitive, Epitopes, B-Lymphocyte/immunology, Hepatitis B Antibodies/*immunology, Hepatitis B Surface Antigens/*immunology, Hepatitis B Vaccines/*immunology, Hepatitis B/*prevention & control, Humans, Immunization, Immunization, Secondary, Solutions, Vaccines, Synthetic/*immunology
dx.doi.org/10.1002/jmv.2146, hdl.handle.net/1765/3848
Journal of Medical Virology
Erasmus MC: University Medical Center Rotterdam

Heijtink, R.A, Kruining, J, van Bergen, P.A.C, de Rave, S, van Hattum, J, Schutten, M, & Osterhaus, A.D.M.E. (2002). Characterization of a human monoclonal antibody obtained after immunization with plasma vaccine and a booster with recombinant-DNA hepatitis B vaccine. Journal of Medical Virology, 66(3), 304–311. doi:10.1002/jmv.2146