Rationale: Recent observations of abnormal immunoglobulin responses and case reports describing successful B-cell ablative therapy suggest involvement ofBcells in the pathogenesis of sarcoidosis. Objectives: To investigate how abnormal B-cell maturation and function in patients with sarcoidosis contribute to disease. Methods: Patients with sarcoidosis (n = 32) were included for detailed analysis by immunohistochemistry of tissue, flow cytometry of blood B-cell subsets, andserumimmunoglobulin levels.Vaccinationresponses in patients with sarcoidosis to influenza virus and encapsulated bacteria andmolecular analysis ofimmunoglobulin heavy chain transcriptswere studied for functional analysis of immunoglobulin responses. Measurements and Main Results: Perigranuloma localization of IgAproducing plasma cells and numerous B cells were found in affected tissues. Total blood B-cell numbers were normal, CD271 memory B cells were significantly reduced, and CD272IgA1 B cells were significantly increased; the results are normalized in patients treated with TNF-ablockers.Despitethis,patientshadnormal serumimmunoglobulin levels and normal antigen-specific immunoglobulin responses. IgAand IgGtranscripts, however, showed highfrequencies of somatic hypermutations and increased usage of downstream IgG subclasses, suggestive for prolonged or repetitive responses. Conclusions: The large B-cell infiltrates in granulomatous tissue and increased molecular signs of antibody maturation are indicative of direct involvement of B cells in local inflammatory processes in patients with sarcoidosis. Moreover, CD272IgA1 B cells could be a marker for treatment with TNF-a blockers. These findings of B cells as emerging key players provide a rationale for a systematic study on B-cell ablative therapy in patients with sarcoidosis. Copyright

Additional Metadata
Keywords Immunoglobulin, Somatic hypermutation, TNF-a, Vaccination response
Persistent URL dx.doi.org/10.1164/rccm.201206-1024OC, hdl.handle.net/1765/38970
Journal American Journal of Respiratory and Critical Care Medicine
Citation
Kamphuis, L.S.J, van Zelm, M.C, Lam, K.H, Rimmelzwaan, G.F, Baarsma, G.S, Dik, W.A, … van Laar, J.A.M. (2013). Perigranuloma localization and abnormal maturation of B cells emerging key players in sarcoidosis?. American Journal of Respiratory and Critical Care Medicine, 187(4), 406–416. doi:10.1164/rccm.201206-1024OC