Background: The hematocrit effect is a hurdle for successful introduction of the dried blood spot (DBS) in a regulated environment. Recently, attempts were taken to overcome the hematocrit effect by whole-cut DBS analysis. This paper presents the next-generation whole-cut DBS; dried matrix on paper disks (DMPD). Results: DMPD eliminated the hematocrit effect and demonstrated better accuracy and precision than regular DBS with partial punching. Observed accuracy and precision were 6.0 and 2.3% for DMPD, respectively, and-10.4 and 17.1%, for DBS, respectively. Conclusion: The DMPD technique performed better than regular DBS by eliminating the hematocrit effect related blood volume bias. Although this effect was not observed with DMPD, a systematic error of 6.0% was detected and further technical development of DMPD could improve the performance.

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doi.org/10.4155/bio.12.175, hdl.handle.net/1765/39052
Bioanalysis
Erasmus MC: University Medical Center Rotterdam

Meesters, R., Zhang, J., van Huizen, N., Hooff, G., Gruters, R., & Luider, T. (2012). Dried matrix on paper disks: The next generation DBS microsampling technique for managing the hematocrit effect in DBS analysis. Bioanalysis, 4(16), 2027–2035. doi:10.4155/bio.12.175