A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.

Bagg albino mouse, RNA virus, Rabies virus, animal, animal cell, animal experiment, animal model, animal tissue, antigen expression, brain, cell inclusion, classification, controlled study, duvenhage virus, female, genetics, hamster, human, human cell, human tissue, immunology, in vitro study, in vivo study, isolation and purification, molecular genetics, mouse, nerve cell necrosis, newborn, nonhuman, nucleotide sequence, pathogenicity, pathology, rabies, rhabdovirus infection, sequence analysis, travel, tumor cell line, virology, virus culture, virus encephalitis, virus isolation, virus pathogenesis, virus virulence
dx.doi.org/10.1371/journal.ppat.1002682, hdl.handle.net/1765/39104
PL o S Pathogens (Online)
Erasmus MC: University Medical Center Rotterdam

Koraka, P, Martina, B.E.E, Roose, J.M, van Thiel, P.P.A.M, van Amerongen, G, Kuiken, T, & Osterhaus, A.D.M.E. (2012). In vitro and in vivo isolation and characterization of Duvenhage virus. PL o S Pathogens (Online), 8(5). doi:10.1371/journal.ppat.1002682