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V. Corman (Vinciane), I. Eckerle, A.M. Zaki (Ali), O. Landt, M. Eschbach-Bludau (Monika), S. van Boheemen (Sander), R. Gopal (Robin), M. Ballhouse, T.M. Bestebroer (Theo), D. Muth, et al. M.A. Müller, J.-F. Drexler (Jan-Felix), M. Zambon, A.D.M.E. Osterhaus (Albert), R.A.M. Fouchier (Ron) and C. Drosten (Christian)

2012-09-01

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

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We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Additional Metadata
Keywords Coronavirus, animal cell, article, controlled study, e gene, gene, human, limit of detection, major clinical study, nonhuman, nucleotide sequence, open reading frame, real time polymerase chain reaction, respiratory virus, reverse transcription polymerase chain reaction, screening, sensitivity and specificity, sputum analysis, virus detection, virus replication
Persistent URL hdl.handle.net/1765/39251
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Corman, V., Eckerle, I., Zaki, A., Landt, O., Eschbach-Bludau, M., van Boheemen, S., … Drosten, C. (2012). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Retrieved from http://hdl.handle.net/1765/39251


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