Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
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|Organisation||Erasmus MC: University Medical Center Rotterdam|
Corman, V, Eckerle, I, Zaki, A.M, Landt, O, Eschbach-Bludau, M, van Boheemen, S, … Drosten, C. (2012). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Retrieved from http://hdl.handle.net/1765/39251