Effectiveness of quenchers to reduce radiolysis of 111- or 177-labelled methionine-containing regulatory peptides. maintaining radiochemical purity as measured by HPLC
Current Topics in Medicinal Chemistry , Volume 12 - Issue 23 p. 2677- 2685
An overview how to measure and to quantify radiolysis by the addition of quenchers and to maintain Radio-Chemical Purity (RCP) of vulnerable methionine-containing regulatory peptides is presented. High RCP was only achieved with a combination of quenchers. However, quantification of RCP is not standardized, and therefore comparison of radiolabelling and RCP of regulatory peptides between different HPLC-systems and between laboratories is cumbersome. Therefore we suggest a set of standardized requirements to quantify RCP by HPLC for radiolabelled DTPA- or DOTA-peptides. Moreover, a dosimetry model was developed to calculate the doses in the reaction vials during radiola-belling and storage of the radiopeptides, and to predict RCP in the presence and absence of quenchers. RCP was measured by HPLC, and a relation between radiation dose and radiolysis of RCP was established. The here described quenchers are tested individually as f(concentration) to investigate efficacy to reduce radiolysis of radiolabelled methionine-containing regulatory peptides. © 2012 Bentham Science Publishers.
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|Current Topics in Medicinal Chemistry|
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de Blois, E, Chan, H.S, Konijnenberg, M, Zanger, R, & Breeman, W.A.P. (2012). Effectiveness of quenchers to reduce radiolysis of 111- or 177-labelled methionine-containing regulatory peptides. maintaining radiochemical purity as measured by HPLC. Current Topics in Medicinal Chemistry, 12(23), 2677–2685. doi:10.2174/1568026611212230005