Enteric administration of a live attenuated measles vaccine does not induce protective immunity in a macaque model
To test the option of oral vaccination with a live attenuated measles vaccine (LAV), we have evaluated the potential of an orally administered enteric-coated tablet containing a candidate LAV (strain Leningrad-16, MV-L16). To this end three groups of two cynomolgus macaques each were vaccinated via different routes with 103.8TCID50MV-L16 vaccine: intramuscularly (i.m.), intraintestinally (i.i.) upon laparotomy and via enteric-coated tablets. Upon vaccination, MV-L16 could only be isolated from one of the i.m.-vaccinated monkeys and not from any of the other five. Both the i.m.-infected monkeys and one of the i.i.-infected monkeys developed a MV-specific serum antibody response. Also, MV-specific CD8+IFNγ-producing T cells could be demonstrated in all three monkeys that had seroconverted. Upon challenge with wild-type MV 1 year after vaccination, only these three monkeys proved to be protected. These data do not support the viability of the concept of oral vaccination with LAVs.
|Keywords||Enteric administration, Macaque model, Measles vaccine|
|Persistent URL||dx.doi.org/10.1016/S0264-410X(02)00231-1, hdl.handle.net/1765/39710|
Stittelaar, K.J, de Swart, R.L, Vos, H.W, van Amerongen, G, Agafonov, A.P, Nechaeva, E.A, & Osterhaus, A.D.M.E. (2002). Enteric administration of a live attenuated measles vaccine does not induce protective immunity in a macaque model. Vaccine, 20(23-24), 2906–2912. doi:10.1016/S0264-410X(02)00231-1