During the WHO campaign to eradicate measles, accurate discrimination between immune and non-immune individuals will become increasingly important. Due to waning immunity in vaccinated populations, the performance of a measles IgG assay depends mainly on its ability to detect reliably seronegative individuals among many vaccinees with low antibody levels. New serological tests based on recombinant proteins detect only a fraction of the total measles virus (MV) specific antibodies. Therefore, several assays based on recombinant MV-haemagglutinin (ELISA and flow cytometry) or MV-fusion protein (flow cytometry) as well as neutralisation and haemagglutination test have been evaluated using a large panel of low-titre and negative sera. Since such an evaluation is highly dependent on threshold values for positivity, the receiver operating characteristic curve analysis was applied. The H-FACS and the H-ELISA showed the best performing characteristics (specificity: 97.4 and 96.1%, respectively; sensitivity: 88.1 and 89.6%, respectively) and may be an alternative to the neutralisation assay. The number of undefined/grey zone sera was significantly lower compared to a commercial whole virus-based ELISA and therefore fewer individuals would be vaccinated unnecessarily. Copyright (C) 2000 Elsevier Science B.V.

Additional Metadata
Keywords ELISA, Flow cytometry, Haemagglutination assay, IgG antibodies, Measles, Neutralisation assay
Persistent URL dx.doi.org/10.1016/S0166-0934(99)00143-3, hdl.handle.net/1765/39754
Journal Journal of Virological Methods
Citation
Hartter, H.K, de Swart, R.L, Hanses, F, Vos, H.W, Bouche, F.B, Osterhaus, A.D.M.E, … Muller, C.P. (2000). Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera. Journal of Virological Methods, 84(2), 191–200. doi:10.1016/S0166-0934(99)00143-3