Wiskott-Aldrich syndrome protein-mediated actin dynamics control type-I interferon production in plasmacytoid dendritic cells
Mutations in Wiskott-Aldrich syndrome (WAS) protein (WASp), a regulator of actin dynamics in hematopoietic cells, cause WAS, an X-linked primary immunodeficiency characterized by recurrent infections and a marked predisposition to develop autoimmune disorders. The mechanisms that link actin alterations to the autoimmune phenotype are still poorly understood. We show that chronic activation of plasmacytoid dendritic cells (pDCs) and elevated type-I interferon (IFN) levels play a role in WAS autoimmunity. WAS patients display increased expression of type-I IFN genes and their inducible targets, alteration in pDCs numbers, and hyperresponsiveness to TLR9. Importantly, ablating IFN-I signaling in WASp null mice rescued chronic activation of conventional DCs, splenomegaly, and colitis. Using WASp-deficient mice, we demonstrated that WASp null pDCs are intrinsically more responsive to multimeric agonist of TLR9 and constitutively secrete type-I IFN but become progressively tolerant to further stimulation. By acute silencing of WASp and actin inhibitors, we show that WASp-mediated actin polymerization controls intracellular trafficking and compartmentalization of TLR9 ligands in pDCs restraining exaggerated activation of the TLR9-IFN-α pathway. Together, these data highlight the role of actin dynamics in pDC innate functions and imply the pDC-IFN-α axis as a player in the onset of autoimmune phenomena in WAS disease.
|Keywords||Wiskott Aldrich syndrome, actin polymerization, animal experiment, article, autoimmune disease, autoimmunity, controlled study, cytokine production, disease predisposition, gene silencing, hematopoietic cell, innate immunity, mouse, nonhuman, plasmacytoid dendritic cell, priority journal, protein function, recurrent infection, signal transduction|
|Persistent URL||dx.doi.org/10.1084/jem.20120363, hdl.handle.net/1765/40866|
|Journal||The Journal of Experimental Medicine|
Prete, F, Catucci, M, Labrada, M, Gobessi, S, Castiello, M.C, Bonomi, E, … Benvenuti, F. (2013). Wiskott-Aldrich syndrome protein-mediated actin dynamics control type-I interferon production in plasmacytoid dendritic cells. The Journal of Experimental Medicine, 210(2), 355–374. doi:10.1084/jem.20120363