2013-10-01
MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia
Publication
Publication
Leukemia Research: clinical and laboratory studies , Volume 37 - Issue 10 p. 1315- 1321
MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4%), miR-125b/miR-100 (93 ± 5%) or miR-125b/miR-99a/miR-100 (82 ± 17%) compared with miR-125b-transduced cells (38 ± 13%, P < 0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p < 0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p < 0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.
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| doi.org/10.1016/j.leukres.2013.06.027, hdl.handle.net/1765/40921 | |
| Leukemia Research: clinical and laboratory studies | |
| Organisation | Erasmus MC: University Medical Center Rotterdam |
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Akbari Moqadam, F., Lange-Turenhout, E., Ariës, I., Pieters, R., & den Boer, M. (2013). MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia. Leukemia Research: clinical and laboratory studies, 37(10), 1315–1321. doi:10.1016/j.leukres.2013.06.027 |
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