The aim of this work is to determine if lectins can be used in "sandwich" ELISA techniques so that the glycosylation of specific proteins in mixtures could be characterised in a fast and sensitive way without prior purification of the protein. Furthermore, the feasability of lectin-enzyme binding assays will be explored in various ways to assess the lectin specificity, and to detect the binding of lectins to normal plasma glycoproteins and their disease-associated glycoforms

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Erasmus University Rotterdam
B. Leijnse
hdl.handle.net/1765/50998
Erasmus MC: University Medical Center Rotterdam

Pekelharing, J. M. (1989, January 18). Lectin-enzyme binding assays : development of the technique and applications in biochemistry and medicine. Retrieved from http://hdl.handle.net/1765/50998