From the introduction, it will be clear that liver lipase is involved in the metabolism of lipoproteins. The exact function(s) of the enzyme in lipoprotein metabolism, however, still remains to be elucidated which of course is necessary for the understanding of a possible relationship between liver lipase activity and regression of atherosclerosis (13). Basic research on the enzyme is needed to reveal the properties of the protein itself. In this study, research is carried out on the molecular structure, the localizaton and the function of the lipase from the liver and the liver-type lipases that are present in the adrenal gland and in the ovary of the rat. Earlier, antibodies against the liver enzyme had been developed. Such antibodies must be monospecific for the liver enzyme, well characterized and be usable in sufficient amounts. Because of these considerations and others (see below), the isolation of a monoclonal antibody against the rat liver enzyme has been undertaken. As compared with the conventional polyclonal antisera, a monoclonal antibody may have certain advantages. In theory, a hybridoma cell line can be a long lasting source of a wellcharacterized antibody. Inhibition of lipase activity by a monoclonal antibody might reveal how the enzyme is capable in hydrolyzing different substrates. When using a polyclonal antiserum for enzyme inhibition, all activity is completely blocked, most probably because of the interaction of several antibodies with the lipase. A monoclonal antibody may be useful in localization studies as the problem of cross-reactivity is often less compared with a polyclonal antibody. For the production and isolation of monoclonal antibodies, immunological assays must be developed and adapted for the reliable, efficient and quick detection of anti-liver lipase antibodies in hundreds of hybridoma supernatants

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Erasmus University Rotterdam
W.C. Hülsmann
hdl.handle.net/1765/51177
Erasmus MC: University Medical Center Rotterdam

Persoon, N. (1987, May 20). Heparin releasable liver-type lipases of the rat : application of monoclonal antibodies. Retrieved from http://hdl.handle.net/1765/51177