1994-08-30
Distinct α1-adrenergic agonist- and endothelin-1-evoked phosphoinositide cycle responses in cultured neonatal rat cardiomyocytes
Publication
Publication
Biochemical and Biophysical Research Communications , Volume 203 - Issue 1 p. 422- 429
Previously it was shown by us and others in cultured neonatal rat cardiomyocytes that the desensitization of the phenylephrine (PHE)- and endothelin-1 (ET-1)-mediated response of phospholipase C (PLC) was receptor-specific. The aim of this study was to characterize receptor-dependent specificities downstream of PLC. PHE (10−4 M) as well as ET-1 (10−8 M) stimulated the total [3H]inositolphosphate ([3H]InsPn, predominantly [3H-3]Ins4P) formation to about the same extent whereas Ins(1,4,5)P3 and Ins(1,3,4,5)P4 did not increase. Yet, ET-1 but not PHE stimulated Ins(1,3,4)P3, and Ins(3,4)Pn, formation. Activation of PLC in saponin-permeabilized cells by GTPγS- and Ca2+ gave predominantly the formation of Ins(1,4)P2. The PHE- and ET-1-mediated increase of [3H]1,2-diacylglycerol was significant after respectively 16 and 8 min. PHE but not ET-1 stimulated phosphorylation of a 30 kDa protein which was likely of myofibrillar origin. It is concluded that receptor-dependent specificities exist not only at the level of PLC but also downstream.
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| doi.org/10.1006/bbrc.1994.2199, hdl.handle.net/1765/54061 | |
| Biochemical and Biophysical Research Communications | |
| Organisation | Department of Biochemistry |
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de Jonge, H., van Heugten, H., Bezstarosti, K., & Lamers, J. (1994). Distinct α1-adrenergic agonist- and endothelin-1-evoked phosphoinositide cycle responses in cultured neonatal rat cardiomyocytes. Biochemical and Biophysical Research Communications, 203(1), 422–429. doi:10.1006/bbrc.1994.2199 |
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