PURPOSE: To investigate whether the peeled internal limiting membrane (ILM) contains cellular retinal cell fragments, and to learn more about their possible origin. DESIGN: Experimental study. METHODS: ILM peeled from ten eyes during vitrectomy by infracyanine green (ICG) was studied immunohistochemically using the markers: GFAP, S-100, and vimentin. Five ILM specimens were from eyes with diabetic macular edema (DME), two from eyes with a macular hole, and three from eyes with persisting macular edema after retinal detachment surgery. RESULTS: In eight of the ten ILM specimens, we found GFAP-positive staining, indicating the presence of remnants of footplates from Müller cells or glial cells. Two ILM specimens were positive for S-100, indicating the presence of neural cells or ganglion cells. CONCLUSIONS: ILM peeled from the retina during vitrectomy using ICG may contain remnants of Müller cell footplates, neural cells, and ganglion cells.

doi.org/10.1016/j.ajo.2005.05.054, hdl.handle.net/1765/56160
American Journal of Ophthalmology
Department of Pathology

La Heij, E. C., Dieudonné, S., Mooy, C., Diederen, R., Liem, A., van Suylen, R.-J., & Hendrikse, F., Prof.Dr. (2005). Immunohistochemical analysis of the internal limiting membrane peeled with infracyanine green. American Journal of Ophthalmology, 140(6), 1123–1125. doi:10.1016/j.ajo.2005.05.054