Isometric contraction of dupuytren's myofibroblasts is inhibited by blocking intercellular junctions
The Journal of Investigative Dermatology , Volume 133 - Issue 12 p. 2664- 2671
Myofibroblasts (MFs) are responsible for both physiological wound and scar contraction. However, it is not known whether these cells act individually to contract the surrounding matrix or whether they behave in a coordinated manner. Therefore, we studied intercellular junctions of primary human MFs derived from patients with Dupuytren's disease, a fibrotic disorder of the dermis and subdermal tissues of the palm. The cells were maintained in anchored three-dimensional collagen lattices to closely mimic conditions in vivo. We found that selective blockade of adherens, mechanosensitive, or gap junctions effectively inhibited contraction of the collagen matrices and downregulated the MF phenotype. Our data indicate that MFs in part function as a coordinated cellular syncytium, and disruption of intercellular communication may provide a therapeutic target in diseases characterized by an overabundance of these contractile cells.
|The Journal of Investigative Dermatology|
|Organisation||Department of Plastic and Reconstructive Surgery|
Verhoekx, J.S.N, Verjee, L.S, Izadi, D, Chan, J.K.K, Nicolaidou, V, Davidson, D, … Nanchahal, J. (2013). Isometric contraction of dupuytren's myofibroblasts is inhibited by blocking intercellular junctions. The Journal of Investigative Dermatology, 133(12), 2664–2671. doi:10.1038/jid.2013.219