2014
Novel vaccine regimen elicits strong airway immune responses and control of respiratory syncytial virus in nonhuman primates
Publication
Publication
Journal of Virology , Volume 88 - Issue 8 p. 3997- 4007
Induction of long-lasting immunity against viral respiratory tract infections remains an elusive goal. Using a nonhuman primate model of human respiratory syncytial virus (hRSV) infection, we compared mucosal and systemic immune responses induced by different DNA delivery approaches to a novel parenteral DNA prime-tonsillar adenoviral vector booster immunization regimen. Intramuscular (i.m.) electroporation (EP) of a DNA vaccine encoding the fusion protein of hRSV induced stronger systemic immune responses than intradermal EP, tattoo immunization, and conventional i.m. DNA injection. A single EP i.m., followed by two atraumatic tonsillar immunizations with the adenoviral vector, elicited strong systemic immune responses, an unique persistent CD4+ and CD8+ T cell response in the lower respiratory tract and protection from intranasal hRSV challenge. Thus, parenteral DNA priming followed by booster immunization targeted to a mucosal inductive site constitutes an effective vaccine regimen for eliciting protective immune responses at mucosal effector sites.
Additional Metadata | |
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doi.org/10.1128/JVI.02736-13, hdl.handle.net/1765/59054 | |
Journal of Virology | |
Organisation | Department of Virology |
Grunwald, T., Tenbusch, M., Schulte, R., Raue, K., Wolf, H. U., Hannaman, D., … Stahl-Hennig, C. (2014). Novel vaccine regimen elicits strong airway immune responses and control of respiratory syncytial virus in nonhuman primates. Journal of Virology, 88(8), 3997–4007. doi:10.1128/JVI.02736-13 |