The effect of hydrocortisone (HC) on PDGF β-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF β-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF β-receptor gene. The expression of PDGF β-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

dx.doi.org/10.1016/S0014-4827(05)80074-6, hdl.handle.net/1765/59587
Experimental Cell Research: emphasizing molecular approaches to cell biology
Immunology

Versnel, M.A, Bouts, A.H, Langerak, A.W, van der Kwast, Th.H, Hoogsteden, H.C, Hagemeijer, A, & Heldin, C.-H. (1992). Hydrocortisone-induced increase of PDGF β-receptor expression in a human malignant mesothelioma cell line. Experimental Cell Research: emphasizing molecular approaches to cell biology, 200(1), 83–88. doi:10.1016/S0014-4827(05)80074-6