We have recently developed a model in which mice were treated with 1L-4 after primary immunization, resulting in elevated total serum IgG1 and IgE levels, but decreased antigen-specific levels and memory formation for these isotypes. In this report, we describe that these effects of IL-4 are mediated at the B cell and not the T-cell level. Major changes occurred in the γ1ɛ-double positive B-cell population which is increased as a result of IL-4 treatment. Moreover, it is shown that γ1ɛ-double positive B cells can develop in vitro out of γ1-positive primed B cells and that these double positive cells can differentiate into IgG1- and IgE-secreting cells. The existence of γ1ɛ-double positive memory B cells can explain the differences in cytokine dependence of TNP-specific memory IgG1 and IgE responses found after adoptively transferring primed spleen cells into irradiated naive recipients. Whereas the IL-4 independent TNP-specific memory IgG1 responses could be blocked efficiently by neutralizing IL-5 and IL-6, TNP-specific memory IgE responses were virtually not susceptible to such treatment. These IgE responses were also not susceptible to IFN-γ, used in doses that could inhibit the primary IgE response. Inhibition of the TNP-specific memory IgG1 response by neutralizing IL-5 and IL-6 is accompanied by a 10-fold increase of the IL-4 independent TNP-specific IgE memory response. These data indicate that secondary IgE responses primarily result from B cells that are either switched to IgG1, or are double positive for IgG1 and IgE, thereby suggesting a minor role for ɛ-single positive B cells in secondary IgE responses.

dx.doi.org/10.1111/j.1365-3083.1994.tb03495.x, hdl.handle.net/1765/60303
Scandinavian Journal of Immunology

van Ommen, R, Vredendaal, A.E.C.M, & Savelkoul, H.F.J. (1994). Secondary IgE responses in vivo are predominantly generated via γ1ε-double positive B cells. Scandinavian Journal of Immunology, 40(5), 491–501. doi:10.1111/j.1365-3083.1994.tb03495.x