BACKGROUND: Mylar balloons are used to collect exhaled air for analysis of fractional nitric oxide concentration (FENO). Aim: We studied the effect of storage conditions on the stability of nitric oxide (NO) in mylar balloons. Methods: Exhaled air samples and calibration gases were stored in mylar balloons at 4, 21 and 37°C, with or without silica gel. NO was measured after 0, 6, 9, 24 and 48 h. Scheffe F-tests were used to compare NO values. Results: NO remained stable in balloons for 9 h at all temperatures, without silica gel. NO increased between 9 and 48 h, but only with low initial FENO. Silica gel increased variability. Conclusions: FENO in mylar balloons is stable for at least 9 h. The storage temperature is not critical, but silica gel increases variability.

Exhaled nitric oxide, Mylar balloons, Silica gel, Stability, Temperature
dx.doi.org/10.1080/0962935031000096971, hdl.handle.net/1765/60618
Mediators of Inflammation
Department of Pediatrics

Bodini, A, Pijnenburg, M.W.H, Boner, A.L, & de Jongste, J.C. (2003). Exhaled nitric oxide in mylar balloons: Influence of storage time, humidity and temperature. Mediators of Inflammation, 12(1), 47–49. doi:10.1080/0962935031000096971