Three molecular tools, amplified fragment length polymorphism (AFLPTm), denaturing gradient gel electrophoresis (DGGE) and random amplified polymorphic DNA (RAPD) analysis, were explored for their usefulness to identify isolates of Malassezia yeasts. All seven species could be separated by AFLP and DGGE. Using AFLP, four genotypes could be distinguished within M. furfur. AFLP genotype 4 contained only isolates from deep human sources, and ca. 80% of these isolates were from patients with systemic disease. Most of the systemic isolates belonged to a single RAPD genotype. This suggests that systemic conditions strongly select for a particular genotype. Although the clinical use of DGGE may be limited due to technical demands, it remains a powerful tool for the analysis of complex clinical samples.

Amplified fragment length polymorphism, Denaturing gradient gel electrophoresis, Identification, Malassezia, Random amplified polymorphic DNA, Yeast,
FEMS Yeast Research
Department of Medical Microbiology and Infectious Diseases

Theelen, B, Silvestri, M, Guého, E, van Belkum, A.F, & Boekhout, T. (2001). Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLPTm), random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE). FEMS Yeast Research, 1(2), 79–86. doi:10.1016/S1567-1356(01)00015-0