Small noncoding differentially methylated copy-number variants, including IncRNA genes, cause a lethal lung developmental disorder
An unanticipated and tremendous amount of the noncoding sequence of the human genome is transcribed. Long noncoding RNAs (IncRNAs) constitute a significant fraction of non-protein-coding transcripts; however, their functions remain enigmatic. We demonstrate that deletions of a small noncoding differentially methylated region at 16q24.1, including IncRNA genes, cause a lethal lung developmental disorder, alveolar capillary dysplasia with misalignment of pulmonary veins (ACD/MPV), with parent-of-origin effects. We identify overlapping deletions 250 kb upstream of FOXF1 in nine patients with ACD/MPV that arose de novo specifically on the maternally inherited chromosome and delete lung-specific IncRNAgenes. These deletions define a distant cis-regulatory region that harbors, besides lncRNAgenes, also a differentially methylated CpGisland, binds GLI2 depending on the methylation status of this CpG island, and physically interacts with and up-regulates the FOXF1 promoter. Wesuggest that lung-transcribed 16q24.1 IncRNAs may contribute to long-range regulation of FOXF1 by GLI2 and other transcription factors. Perturbation of IncRNA-mediated chromatin interactions may, in general, be responsible for position effect phenomena and potentially cause many disorders of human development.
|Persistent URL||dx.doi.org/10.1101/gr.141887.112, hdl.handle.net/1765/61472|
Szafranski, P, Dharmadhikari, A.V, Brosens, E, Gurha, P, Kołodziejska, K.E, Zhishuo, O, … Stankiewicz, P. (2013). Small noncoding differentially methylated copy-number variants, including IncRNA genes, cause a lethal lung developmental disorder. Genome Research, 23(1), 22–33. doi:10.1101/gr.141887.112