In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-1 (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen.

Fibrin, Fibrinogen, Hepassocin, Hepatocellular carcinoma, Hepatocyte-derived fibrinogen-related protein-1, Plasma clot, Proteomics, Regulation of cell growth,
Biochemical and Biophysical Research Communications
Department of Neurology

Rijken, D.C, Dirkx, S.P.G, Luider, T.M, & Leebeek, F.W.G. (2006). Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot. Biochemical and Biophysical Research Communications, 350(1), 191–194. doi:10.1016/j.bbrc.2006.09.018