2006-11-10
Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot
Publication
Publication
Biochemical and Biophysical Research Communications , Volume 350 - Issue 1 p. 191- 194
In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-1 (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen.
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| doi.org/10.1016/j.bbrc.2006.09.018, hdl.handle.net/1765/61586 | |
| Biochemical and Biophysical Research Communications | |
| Organisation | Department of Neurology |
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Rijken, D., Dirkx, S. P. G., Luider, T., & Leebeek, F. (2006). Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot. Biochemical and Biophysical Research Communications, 350(1), 191–194. doi:10.1016/j.bbrc.2006.09.018 |
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