Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures

Mendes, Filipa; Doucet, Laurent; Hinzpeter, Alexandre; Féree, Claude; Lipecka, Joanna; Fritsch, Janine; Edelman, David; Jorna, Huub; Willemsen, Rob; Bot, Alice; de Jonge, Hugo; Hinnrasky, Jocelyne; Castillon, Nicolas; Taouil, Karima; Puchelle, Edith; Penque, Deborah; Amaral, Margarida

doi:10.1016/j.jcf.2004.05.008

Studies on CFTR protein expression and localization in native tissues or in primary cultures of human epithelial cells are scarce due to the intrinsic instability of this protein, its low expression in most tissues and also to technical difficulties.However, such data are of the highest importance to understand the pathophysiology of CF. The purpose of this article is to outline several assays for the characterization of primary epithelial cultures and to review different CFTR immunostaining protocols.

Additional Metadata
Keywords	Anti-CFTR antibodies, Immunohistochemistry
Persistent URL	doi.org/10.1016/j.jcf.2004.05.008, hdl.handle.net/1765/61702
Journal	Journal of Cystic Fibrosis
Organisation	Department of Clinical Genetics
Citation APA Style AAA Style APA Style Cell Style Chicago Style Harvard Style IEEE Style MLA Style Nature Style Vancouver Style American-Institute-of-Physics Style Council-of-Science-Editors Style BibTex Format Endnote Format RIS Format CSL Format DOIs only Format	Mendes, F., Doucet, L., Hinzpeter, A., Féree, C., Lipecka, J., Fritsch, J., … Amaral, M. (2004). Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures. Journal of Cystic Fibrosis, 3(SUPPL. 2), 37–41. doi:10.1016/j.jcf.2004.05.008

Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures

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Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures

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