In this study, we investigated the expression of lipocortin I and II (annexin I and II) in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-lß, TNF-α or LPS for 24h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGFla production was significantly increased. This DL-lß- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

Bronchial epithelial cells, Eicosanoids, Glucocorticoids, Lipocortins
dx.doi.org/10.1155/S0962935196000300, hdl.handle.net/1765/61783
Mediators of Inflammation
Department of Pharmacology

Verheggen, M.M, de Bont, H.I.M, Adriaansen-Soeting, P.W.C, Goense, B.J.A, Tak, C.J.A.M, Hoogsteden, H.C, … Versnel, M.A. (1996). Expression of lipocortins in human bronchial epithelial cells: Effects of IL-1β, TNF-α, LPS and dexamethasone. Mediators of Inflammation, 5(3), 210–217. doi:10.1155/S0962935196000300