Background: Because persistent inflammation plays a dominant role in cystic fibrosis (CF), we assessed systemic and local upper airway responses during and after pulmonary exacerbation. Methods: We followed a cohort of Pseudomonas aeruginosa-infected adult CF patients (n = 16) over time in pulmonary exacerbation and in stable disease. Interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A, IL-22, interferon-γ and TNFα levels were measured in sputum, nasal lavages and plasma. Results: In CF patients IL-6 and IL-10 levels in nasal lavages were significantly increased in exacerbation compared with stable disease. Systemic IL-6 significantly correlated with CRP levels and FEV1 (%predicted), independently of disease status. Systemic IL-10 also correlated significantly with CRP and FEV1 (%predicted), but only in exacerbation. Other cytokines tested did not discriminate between exacerbation and stable disease. Conclusions: Determination of IL-6 and IL-10 in nasal lavages may provide a minimally invasive tool in the assessment of an exacerbation in CF.

Cystic fibrosis, Cytokines, Inflammation, Interleukin, Nasal lavages,
Journal of Cystic Fibrosis
Department of Immunology

Paats, M.S, Bergen, I.M, Bakker, M, Hoek, R.A.S, Nietzman-Lammering, K.J, Hoogsteden, H.C, … van der Eerden, M. (2013). Cytokines in nasal lavages and plasma and their correlation with clinical parameters in cystic fibrosis. Journal of Cystic Fibrosis, 12(6), 623–629. doi:10.1016/j.jcf.2013.05.002