2006-10-01
In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals
Publication
Publication
European Journal of Nuclear Medicine and Molecular Imaging , Volume 33 - Issue 10 p. 1214- 1217
Purpose: In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats. Methods: Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined. Results: Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r 2=0.946; slope m=1.059). Conclusion: Quantification in vivo using this multi-pinhole SPECT system is highly accurate.
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| doi.org/10.1007/s00259-006-0178-3, hdl.handle.net/1765/62842 | |
| European Journal of Nuclear Medicine and Molecular Imaging | |
| Organisation | Department of Nuclear Medicine |
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Forrer, F., Valkema, R., Bernard, B., Schramm, N., Hoppin, J., Rolleman, E., … de Jong, M. (2006). In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals. European Journal of Nuclear Medicine and Molecular Imaging, 33(10), 1214–1217. doi:10.1007/s00259-006-0178-3 |
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