Purkinje cell-specific ablation of CaV2.1 Channels is sufficient to cause cerebellar ataxia in mice
The Cacna1a gene encodes the α1A subunit of voltage-gated CaV2.1 Ca2+ channels that are involved in neurotransmission at central synapses. CaV2.1-α1- knockout (α1KO) mice, which lack CaV2.1 channels in all neurons, have a very severe phenotype of cerebellar ataxia and dystonia, and usually die around postnatal day 20. This early lethality, combined with the wide expression of CaV2.1 channels throughout the cerebellar cortex and nuclei, prohibited determination of the contribution of particular cerebellar cell types to the development of the severe neurobiological phenotype in Cacna1a mutant mice. Here, we crossed conditional Cacna1a mice with transgenic mice expressing Cre recombinase, driven by the Purkinje cell-specific Pcp2 promoter, to specifically ablate the CaV2.1- α1A subunit and thereby CaV2.1 channels in Purkinje cells. Purkinje cell CaV2.1-α1A-knockout (PCα1KO) mice aged without difficulties, rescuing the lethal phenotype seen in α1KO mice. PCα1KO mice exhibited cerebellar ataxia starting around P12, much earlier than the first signs of progressive Purkinje cell loss, which appears in these mice between P30 and P45. Secondary cell loss was observed in the granular and molecular layers of the cerebellum and the volume of all individual cerebellar nuclei was reduced. In this mouse model with a cell type-specific ablation of CaV2.1 channels, we show that ablation of CaV2.1 channels restricted to Purkinje cells is sufficient to cause cerebellar ataxia. We demonstrate that spatial ablation of CaV2.1 channels may help in unraveling mechanisms of human disease.
|Keywords||Ataxia, Cacna1a, Cell-specific knockout, Conditional, P/Q-type Ca2+ channels|
|Persistent URL||dx.doi.org/10.1007/s12311-011-0302-1, hdl.handle.net/1765/63707|
Todorov, B, Kros, L, Shyti, R, Plak, P, Haasdijk, E.D, Raike, R.S, … Dichgans, M. (2012). Purkinje cell-specific ablation of CaV2.1 Channels is sufficient to cause cerebellar ataxia in mice. The Cerebellum, 11(1), 246–258. doi:10.1007/s12311-011-0302-1