Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of doubleinfection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

dx.doi.org/10.1099/vir.0.042671-0, hdl.handle.net/1765/63760
Journal of General Virology
Department of Virology

Bodewes, R, Nieuwkoop, N.J, Verburgh, R.J, Fouchier, R.A.M, Osterhaus, A.D.M.E, & Rimmelzwaan, G.F. (2012). Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro. Journal of General Virology, 93(8), 1645–1648. doi:10.1099/vir.0.042671-0