The effectiveness of photodynamic therapy is strongly dependent on the availabilty of oxygen. In the present paper we show that the ratio between photosensitiser phosphorescence and fluorescence is a parameter that can be used to monitor the competition between singlet oxygen production and other processes quenching the photosensitiser triplet state. We present a theoretical basis for the validity of this approach and a series of in vitro imaging experiments.

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Journal Optics Express
Sterenborg, H.J.C.M, de Wolf, J.W, Koning, M, Kruijt, B, van der Ploeg-van den Heuvel, A, & Robinson, D.J. (2004). Phosphorescence-Fluorescence ratio imaging for monitoring the oxygen status during photodynamic therapy. Optics Express, 12(9), 1873–1878. doi:10.1364/OPEX.12.001873