We have identified a novel transcript that is abundantly and specifically expressed in both the adult and developing rat CNS. Within the full-length cDNA sequence we were unable to identify a clear open reading frame. Moreover, we were unable to detect any protein product derived from the full-length cDNA sequence using an in vitro translation assay. Therefore, we suggest this gene is one of a growing number of non-coding mRNA-like RNA transcripts that exert their cellular functions directly as an RNA. We have named this novel gene Ntab for non-coding transcript abundantly expressed in brain (accession number AY035551). In addition, in some regions of the brain we find evidence for RNA accumulation in cellular processes at some distance from the soma. These findings suggest that Ntab is actively transported and may function within cellular processes. Since Ntab is a targeted non-coding RNA, such cellular functions could include the targeting and/or regulation of localised translation of other mRNA species.

Cerebellum, Neural gene expression, Non-coding RNA, RNA targeting
dx.doi.org/10.1016/S0306-4522(01)00408-0, hdl.handle.net/1765/64011
Neuroscience
Department of Neuroscience

French, P.J, Bliss, T.V.P, & O'Connor, V. (2001). Ntab, a novel non-coding RNA abundantly expressed in rat brain. Neuroscience, 108(2), 207–215. doi:10.1016/S0306-4522(01)00408-0