1994
Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens
Publication
Publication
European Journal of Immunology , Volume 24 - Issue 10 p. 2279- 2284
The characterization of early branch points in the differentiation of leukocytes requires identification of precursor cells in the bone marrow. Recently, we produced two monoclonal antibodies, ER-MP12 and ER-MP20, which in two-color flow-cytometric analysis divide the murine bone marrow into six defined subsets. Here we show, using fluorescence-activated cell sorting followed by macrophage colony-stimulating factor-stimulated culture in soft agar, that precursors of the mononuclear phagocyte system reside only within the ER-MP12hi20−, ER-MP12+20+ and ER-MP12−20hi bone marrow subsets. Together, these subsets comprise 15% of nucleated bone marrow cells. Furthermore, we provide evidence that the macrophage precursors present in these subsets represent successive stages in a maturation sequence where the most immature ER-MP12hi20− cells develop via the ER-MP12+20+ stage into ER-MP12−20hi monocytes.
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| doi.org/10.1002/eji.1830241003, hdl.handle.net/1765/64174 | |
| European Journal of Immunology | |
| Organisation | Department of Immunology |
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de Bruijn, M., Slieker, W., van der Loo, J. C., Voerman, J., van Ewijk, W., & Leenen, P. (1994). Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens. European Journal of Immunology, 24(10), 2279–2284. doi:10.1002/eji.1830241003 |
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