This paper describes an antibody capture spot-ELISA for the detection of anti-insulin antibody-secreting cells. The assay is based on the binding of secreted antibodies by immobilised isotype-specific capture antibodies and subsequent detection of insulin-specific antibodies with a conjugate of human insulin and alkaline phosphatase (HI-AP). Compared with the conventional approach, using antigen for coating and employing an enzyme-linked detecting antibody, this technique improved the detection of murine cells secreting anti-insulin antibodies of different IgG subclasses.

Anti-insulin antibody, Antibody-secreting cell, ELISA, spot-, IgG isotype
dx.doi.org/10.1016/0022-1759(90)90015-N, hdl.handle.net/1765/64411
Journal of Immunological Methods
Immunology

Vos, Q, Claassen, H.J.H.M, & Benner, R. (1990). Substantially increased sensitivity of the spot-ELISA for the detection of anti-insulin antibody-secreting cells using a capture antibody and enzyme-conjugated insulin. Journal of Immunological Methods, 126(1), 89–94. doi:10.1016/0022-1759(90)90015-N