We have investigated the involvement of activin receptors and TGFβ type I receptor in zebrafish development. Overexpression of either full-length or a truncated form of mouse ActR-IIA interferes with the development. Different splice variants of mouse ActR-IIB have distinct effects; ActR-IIB4 induces abnormal embryos, whereas ActR-IIB2 does not. Activin and TGFβ type I receptors can induce axis duplications. Go-expression of ActR-IA or ActR-IB with the type II activin receptors results in a synergistic increase of the frequency of axis duplication. Moreover, ActR-IIB2 is synergistic with ActR-IA and ActR-IB, demonstrating that ActR-IIB2 can interact with the zebrafish ligand. Overexpression of TGFβR-I with ActR-IIA or ActR-IIB4 results in a synergistic increase in frequency of abnormal embryos, whereas in combination with ActR-IIB2 no such increase occurs.

Activin receptors, Axis formation, Zebrafish
dx.doi.org/10.1016/0925-4773(95)00480-7, hdl.handle.net/1765/64982
Mechanisms of Development
Department of Gastroenterology & Hepatology

de Vries, C.J.M, de Boer, J, Joore, J, Strähle, U, van Achterberg, T.A.E, Huylebroeck, D, … Zivkovic, D. (1996). Active complex formation of type I and type II activin and TGFβ receptors in vivo as studied by overexpression in zebrafish embryos. Mechanisms of Development, 54(2), 225–236. doi:10.1016/0925-4773(95)00480-7