Background: Recent studies have clearly demonstrated the enormous virus diversity that exists among wild animals. This exemplifies the required expansion of our knowledge of the virus diversity present in wildlife, as well as the potential transmission of these viruses to domestic animals or humans. Methods. In the present study we evaluated the viral diversity of fecal samples (n = 42) collected from 10 different species of wild small carnivores inhabiting the northern part of Spain using random PCR in combination with next-generation sequencing. Samples were collected from American mink (Neovison vison), European mink (Mustela lutreola), European polecat (Mustela putorius), European pine marten (Martes martes), stone marten (Martes foina), Eurasian otter (Lutra lutra) and Eurasian badger (Meles meles) of the family of Mustelidae; common genet (Genetta genetta) of the family of Viverridae; red fox (Vulpes vulpes) of the family of Canidae and European wild cat (Felis silvestris) of the family of Felidae. Results: A number of sequences of possible novel viruses or virus variants were detected, including a theilovirus, phleboviruses, an amdovirus, a kobuvirus and picobirnaviruses. Conclusions: Using random PCR in combination with next generation sequencing, sequences of various novel viruses or virus variants were detected in fecal samples collected from Spanish carnivores. Detected novel viruses highlight the viral diversity that is present in fecal material of wild carnivores.

Amdovirus, Bunyavirus, Carnivore, Common gennet, Fox, Otter, Phlebovirus, Picobirnavirus, Red fox, Theilovirus, Viral metagenomics, Wild carnivore, Wildlife
dx.doi.org/10.1186/1743-422X-11-89, hdl.handle.net/1765/65015
Virology Journal
This work was funded by the European Commission 7th Framework Programme; grant id fp7/223498 - European management platform for emerging and re-emerging infectious disease entities (EMPERIE)
Department of Virology

Bodewes, R, Ruiz-Gonzalez, A, Schapendonk, C.M.E, van den Brand, J.M.A, Osterhaus, A.D.M.E, & Smits, S.L. (2014). Viral metagenomic analysis of feces of wild small carnivores. Virology Journal, 11(1). doi:10.1186/1743-422X-11-89