The aim of this study was to evaluate the suitability of tissue-engineered mucosa (TEM) as a model for studying the acute effects of ionizing radiation (IR) on the oral mucosa. TEM and native non-keratinizing oral mucosa (NNOM) were exposed to a single dose of 16.5 Gy and harvested at 1, 6, 24, 48, and 72 h post-irradiation. DNA damage induced by IR was determined using p53 binding protein 1 (53BP1), and DNA repair was determined using Rad51. Various components of the epithelial layer, basement membrane, and underlying connective tissue were analyzed using immunohistochemistry. The expression of cytokines interleukin-1β (IL-1β) and transforming growth factor beta 1 (TGF-β1) was analyzed using an enzyme-linked immunosorbent assay. The expression of DNA damage protein 53BP1 and repair protein Rad51 were increased post-irradiation. The expression of keratin 19, vimentin, collage type IV, desmoglein 3, and integrins α6 and β4 was altered post-irradiation. Proliferation significantly decreased at 24, 48, and 72 h post-irradiation in both NNOM and TEM. IR increased the secretion of IL-1β, whereas TGF-β1 secretion was not altered. All observed IR-induced alterations in TEM were also observed in NNOM. Based on the similar response of TEM and NNOM to IR we consider our TEM construct a suitable model to quantify the acute biological effects of IR.

cytokines, DNA damage, keratinocytes, oral mucosa, tissue engineering
dx.doi.org/10.1016/j.ijom.2013.01.025, hdl.handle.net/1765/65213
International Journal of Oral and Maxillofacial Surgery
Department of Plastic and Reconstructive Surgery

Tra, W.M.W, Tuk, B, van Neck, J.W, Hovius, S.E.R, & Perez-Amodio, S. (2013). Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation. International Journal of Oral and Maxillofacial Surgery, 42(8), 939–948. doi:10.1016/j.ijom.2013.01.025