Host factors determine anti-GM1 response following oral challenge of chickens with Guillain-Barré syndrome derived campylobacter jejuni strain GB11
PLoS ONE , Volume 5 - Issue 3
Background: Anti-ganglioside antibodies with a pathogenic potential are present in C. jejuni-associated Guillain-Barré syndrome (GBS) patients and are probably induced by molecular mimicry. Immunization studies in rabbits and mice have demonstrated that these anti-ganglioside antibodies can be induced using purified lipo-oligosaccharides (LOS) from C. jejuni in a strong adjuvant. Methodology/Principal Findings: To investigate whether natural colonization of chickens with a ganglioside-mimicking C. jejuni strain induces an anti-ganglioside response, and to investigate the diversity in anti-ganglioside response between and within genetically different chicken lines, we orally challenged chickens with different C. jejuni strains. Oral challenge of chickens with a C. jejuni strain from a GBS patient, containing a LOS that mimics ganglioside GM1, induced specific IgM and IgG anti-LOS and anti-GM1 antibodies. Inoculation of chickens with the Penner HS:3 serostrain, without a GM1-like structure, induced anti-LOS but no anti-ganglioside antibodies. We observed different patterns of anti-LOS/ganglioside response between and within the five strains of chickens. Conclusions: Natural infection of chickens with C. jejuni induces anti-ganglioside antibodies. The production of antibodies is governed by both microbial and host factors.
Ang, C.W, Dijkstra, J.R, de Klerk, M.A, Endtz, H.P, van Doorn, P.A, Jacobs, B.C, … Wagenaar, J.A. (2010). Host factors determine anti-GM1 response following oral challenge of chickens with Guillain-Barré syndrome derived campylobacter jejuni strain GB11. PLoS ONE, 5(3). doi:10.1371/journal.pone.0009820