The ability of counter immunoelectrophoresis (CIE) to detectBacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection ofB. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.

Amniotic fluid, B. fragilis, Counterimmunoelectrophoresis, Endotoxin, Ultrafiltration
dx.doi.org/10.1007/BF00882769, hdl.handle.net/1765/65872
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Department of Gynaecology & Obstetrics

Beckmann, I, de Graaff, K, Meisel-Mikolajczyk, F, & Wallenburg, H.C.S. (1994). Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 66(4), 333–336. doi:10.1007/BF00882769