Introduction. Isolated limb perfusion (ILP) with TNFα in combination with melphalan and IFNγ/has resulted in an immediate and dramatic tumor response in patients. Such an effect was also noted following ILP in a rat sarcoma model. This model enables us to investigate several factors responsible for the TNFα-induced tumor responses. We applied total body irradiation (TBI) to reduce white blood cell count, to investigate the contribution of leukocytes to the antitumor effect of TNFα.

Journal of Surgical Research
Department of Surgery

Manusama, E., Nooijen, P. T. G. A., Stavast, J., de Wilt, J., Marquet, R., & Eggermont, A. (1998). Assessment of the role of neutrophils on the antitumor effect of TNFα in an in vivo isolated limb perfusion model sarcoma-bearing brown Norway rats. Journal of Surgical Research, 78(2), 169–175. doi:10.1006/jsre.1997.5256