A generic tool for biotinylation of tagged proteins in transgenic mice
Transgenic Research , Volume 14 - Issue 4 p. 477- 482
The remarkable high affinity (Kd ~ 10 -15 M) of avidin/streptavidin for biotin has been extensively exploited in purification methodologies. Recently a small peptide sequence (Avi-tag) has been defined that can be specifically and efficiently biotinylated by the bacterial BirA biotin ligase. Fusion of this small peptide sequence to a protein of interest and co-expression with the BirA gene in mammalian cells allowed purification of the biotinylated protein together with its associated proteins and other molecules. Ideally, one would like to apply these technologies to purify tagged proteins directly from mouse tissues. To make this approach feasible for a large variety of proteins we developed a mouse strain that expresses the BirA gene ubiquitously by inserting it in the ROSA26 locus. We demonstrate that the BirA protein is indeed expressed in all tissues tested. In order to demonstrate functionality we show that it biotinylates the transgene-encoded Avi-tagged Gata1 and Oct6 transcription factors in erythroid cells of the foetal liver and Schwann cells of the peripheral nerve respectively. Therefore, this mouse can be crossed to any transgenic mouse to obtain efficient biotinylation of an Avi-tagged protein for the purpose of protein (complex) purification.
|Biotinylation, BirA, Gata1, Protein tag, ROSA26|
Driegen, M.J.F, Ferreira, R, van Zon, A, Strouboulis, J, Jaegle, M.M, Grosveld, F.G, … Meijer, D.N. (2005). A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Research, 14(4), 477–482. doi:10.1007/s11248-005-7220-2