In the rat, the extent of collateralization of projections from the cerebellar nuclei to the red nucleus and inferior olive was investigated using a retrograde double labeling technique. The combination of tracers selected, cholera toxin-β-subunit and WGA-BSA-gold, not only enabled the use of small injection sites but also resulted in clearly distinguishable and permanently stained neurons that could be analyzed in counterstained sections.

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Brain Research
Department of Neuroscience

Teune, T.M, van der Burg, J, & Ruigrok, T.J.H. (1995). Cerebellar projections to the red nucleus and inferior olive originate from separate populations of neurons in the rat: A non-fluorescent double labeling study. Brain Research, 673(2), 313–319. doi:10.1016/0006-8993(94)01431-G