Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3-/- mice showed ∼3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were ∼50% lower in UCP3-/- mice, in comparison to UCP3+/+ animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3-/- and UCP3+/+ mice.

High fat diet, Lipotoxicity, Mitochondrion, Reactive oxygen species, UCP3
dx.doi.org/10.1016/j.febslet.2006.01.059, hdl.handle.net/1765/66778
F E B S Letters
Department of Biochemistry

Hoeks, J, Hesselink, M.K.C, Sluiter, W.J, Schaart, G, Willems, J, Morrisson, A, … Schrauwen, P. (2006). The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice. F E B S Letters, 580(5), 1371–1375. doi:10.1016/j.febslet.2006.01.059