2012-09-26
The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences
Publication
Publication
PLoS ONE , Volume 7 - Issue 9
The leukemia-associated fusion protein MN1-TEL combines the transcription-activating domains of MN1 with the DNA-binding domain of the transcriptional repressor TEL. Quantitative photobleaching experiments revealed that ~20% of GFP-tagged MN1 and TEL is transiently immobilised, likely due to indirect or direct DNA binding, since transcription inhibition abolished immobilisation. Interestingly, ~50% of the MN1-TEL fusion protein was immobile with much longer binding times than unfused MN1 and TEL. MN1-TEL immobilisation was not observed when the TEL DNA-binding domain was disrupted, suggesting that MN1-TEL stably occupies TEL recognition sequences, preventing binding of factors required for proper transcription regulation, which may contribute to leukemogenesis.
| Additional Metadata | |
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| doi.org/10.1371/journal.pone.0046085, hdl.handle.net/1765/66882 | |
| PLoS ONE | |
| Organisation | Department of Pathology |
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ter Haar, M., Meester-Smoor, M., van Wely, K., Schot, C. C. M., Janssen, M., Geverts, B., … Zwarthoff, E. (2012). The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences. PLoS ONE, 7(9). doi:10.1371/journal.pone.0046085 |
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