Isoelectric focusing of iron saturated serum has been established as a convenient method for showing transferrin glycan microheterogeneity. In a clinical setting, the method is used in the detection of cerebrospinal fluid leakage, the screening for surreptitious alcohol abuse and in the diagnosis of the carbohydrate deficient glycoprotein syndrome. In normal physiological states it can also be used as a tool to probe for changes in N-glycosylation.

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Glycoconjugate Journal
Department of Clinical Chemistry

de Jong, G, Feelders, R.A, van Noort, W.L, & van Eijk, H.G. (1995). Transferrin microheterogeneity as a probe in normal and disease states. Glycoconjugate Journal, 12(3), 219–226. doi:10.1007/BF00731323