PURPOSE:: The aim of this study was to describe the ultrastructure of the host-donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty. METHODS:: The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation. RESULTS:: Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal "virgin" corneal architecture. CONCLUSIONS:: Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures. Copyright

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doi.org/10.1097/ICO.0000000000000156, hdl.handle.net/1765/67139
Cornea: the journal of cornea and external disease
Department of Ophthalmology

Livny, E, Parker, J.S, van der Kaaij, M, Haasdijk, E.D, van der Wees, J, Bruinsma, M, & Melles, G.R.J. (2014). Postmortem ultrastructural analysis of a cornea transplanted with descemet membrane endothelial keratoplasty. Cornea: the journal of cornea and external disease, 33(8), 790–794. doi:10.1097/ICO.0000000000000156