PURPOSE:: The aim of this study was to describe the ultrastructure of the host-donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty. METHODS:: The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation. RESULTS:: Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal "virgin" corneal architecture. CONCLUSIONS:: Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures. Copyright

corneal transplantation, Descemet membrane, DMEK, electron microscopy, endothelium, ultrastructure
dx.doi.org/10.1097/ICO.0000000000000156, hdl.handle.net/1765/67139
Cornea: the journal of cornea and external disease
Department of Ophthalmology

Livny, E, Parker, J.S, van der Kaaij, M, Haasdijk, E.D, van der Wees, J, Bruinsma, M, & Melles, G.R.J. (2014). Postmortem ultrastructural analysis of a cornea transplanted with descemet membrane endothelial keratoplasty. Cornea: the journal of cornea and external disease, 33(8), 790–794. doi:10.1097/ICO.0000000000000156