Biochemical methods to assess CFTR expression and membrane localization

Farinha, Carlos; Penque, Deborah; Roxo-Rosa, Mónica; Lukacs, Gergely; Dormer, Robert; McPherson, Margaret; Pereira, Malcolm; Bot, Alice; Jorna, Huub; Willemsen, Rob; de Jonge, Hugo; Heda, Ghanshyam; Marino, Christopher; Fanen, Pascale; Hinzpeter, Alexandre; Lipecka, Joanna; Fritsch, Janine; Gentzsch, Martina; Edelman, David; Amaral, Margarida

doi:10.1016/j.jcf.2004.05.017

Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.

Additional Metadata
Keywords	ABC transporter, ABCC7, Biochemical analyses, CFTR, Methods
Persistent URL	doi.org/10.1016/j.jcf.2004.05.017, hdl.handle.net/1765/67818
Journal	Journal of Cystic Fibrosis
Organisation	Department of Clinical Genetics
Citation APA Style AAA Style APA Style Cell Style Chicago Style Harvard Style IEEE Style MLA Style Nature Style Vancouver Style American-Institute-of-Physics Style Council-of-Science-Editors Style BibTex Format Endnote Format RIS Format CSL Format DOIs only Format	Farinha, C., Penque, D., Roxo-Rosa, M., Lukacs, G., Dormer, R., McPherson, M., … Amaral, M. (2004). Biochemical methods to assess CFTR expression and membrane localization. Journal of Cystic Fibrosis, 3(SUPPL. 2), 73–77. doi:10.1016/j.jcf.2004.05.017

Biochemical methods to assess CFTR expression and membrane localization

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Biochemical methods to assess CFTR expression and membrane localization

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